Cell Lines

Products list:




Ready-made Cell Lines

Horizon's isogenic human cell line pairs provide a model system for the study of the role of genes in cellular processes and drug responses. All cell lines are provided with a matched wild type control parental cell line ensuring phenotypes can be attributed directly to the requested genetic modification(s).

Benefits:

  • Confidence - Each cell line is matched with an isogenic control to confirm your results
  • Reliability - Highly characterised and quality controlled clonal human cell lines
  • Convenience - Over 2500 edited cell lines in our catalog ready to be delivered within only a few weeks

Horizon's catalog contains an expanding range of edited cell lines that include common cancer cell lines such as DLD1, MCF10a and HCT116, as well as our proprietary HAP1 cell lines.

Knockout

Advantages of Horizon's gene-edited knockout cell lines:

  • Complete loss of gene function
  • Includes isogenic controls to confirm your results
  • Over 2000 clonal cell lines available for immediate hypothesis testing

Knockin

Disease relevant mutations can be recapitulated or even correct by introducing a point mutation with a single base change, or by inserting/deleting large gene sequences. These endogenous knockin cell lines enable a clear understanding of the contribution of the gene or mutation to a phenotype, in contrast to overexpression models where high expression (either transiently or stably) can lead to false positives. Horizon produces rAAV and CRISPR knockin cell lines or a combination of both.

Reporter

Horizon and Promega Corporation have partnered in a long-term relationship to develop a suite of best-in-class endogenous pathway tag and reporter cell lines for application in functional genomics research and drug discovery and development. Gene-editing at the endogenous level allows researchers to examine near-physiological levels of protein and promoter activity. Traditional methods of understanding protein/promoter function have used exogenous plasmid-based over-expression systems which can result in artefactual results.




Cell Line Engineering

With nearly a decade of experience planning and executing nearly 3,000 gene editing experiments, access to a multiple genome editing technologies, and cell line development service options to suit a variety of experimental needs and budgets, Horizon is the global leader in cell line engineering.

Benefits:

  • Strong IP position to support your freedom to operate
  • Substantial experience with over 100 cell lines and 200 target genes
  • Emphasis on design to test your hypothesis, not just project execution

iPSC

Horizon's leading cell line engineering service now available in human pluripotent stem cells. Induced Pluripotent Stem Cells (iPSC) provide researchers with translational in vitro models that allows study of 'disease in a dish' for difficult to represent diseases and to discover novel drug targets with increased predictability of their safety and efficacy. Gene-editing of iPSCs allows researchers to create isogenic cell models containing key disease driving mutations to achieve mechanistic understanding, without background genetic variability allowing determination of causative relationships between genotype and phenotype.

iPC Cell uses:

  • Knockour a gene of interest
  • Knockin a disease-associated point mutation
  • Reversion to wildtype in disease-derived iPS line
  • Tag a gene of interest with a choice of reporters

Immuno-oncology

Power Your Immune-Oncology Projects with Custom Engineered Immune Cells

Gene-editing in immune cell lines can be challenging due to low targeting efficiency and difficulties in single cell derivation of suspension cells. Horizon has validated 10+ immune cell lines including THP-1, Jurkat and NALM-6 cells for gene-editing projects. CRISPR, rAAV and ZFN gene-editing technologies are available depending on project requirements. Take advantage of the largest panel of pre-characterised immune cell lines available and benefit from Horizon's exceptional knowhow and experience in completing over 2,000 gene-editing projects.

CHO

Horizon provide a service that enables custom optimisation of CHO expression platforms. This allows you to direct the manipulation of the CHO genome to suit your specific requirements. They have successfully engineered thousands of cell lines, including direct experience of CHO. Not only has Horizon generated their own knockout cell line, but they have extensive experience of handling proprietary cell lines. With dedicated lab space and enhanced SOPs to ensure the highest standards, Horizon have become a trusted partner for large pharma and for biotechs wishing to engineer their CHO cells.

Horizon are uniquely placed to provide a range of engineering possibilities. They are truly technology agnostic, and with their wealth of experience in rAAV and nuclease genome editing technologies including CRISPR, Horizon have the flexibility to make almost any modification you may require, ranging from point mutations to large scale translocations. Projects can be based on a cell line provided to them or on one of their commercially available lines, and can be structured in different ways depending on budgetary requirements.




Knockout Cell Lines

Advantages of Horizon's gene-edited knockout cell lines:

  • Complete loss of gene function
  • Includes isogenic controls to confirm your results
  • Over 2000 clonal cell lines available for immediate hypothesis testing

Making Frameshift and Deletion Knockout Cell Lines

Using CRISPR Cas technology Horizon can disable the gene of interest through the introduction of a frameshift mutation or by the removal of large fragments of the gene. Frameshift mutations result in a small insertion or deletion (or InDel), via non-homologous end joining or NHEJ repair pathway in a coding exon. Consequently, this disrupts the gene at the endogenous level. This is a well-established method that results in clonal cell lines where the targeted gene is inactivated.

how_we_make_knockout_diagram_1.pngThe schematic diagram illustrates how Cas9 cleavage can introduce a frameshift mutation (in this case a small deletion) resulting in a non-functional gene.


hap1-ko-western-blot.pngThe absence of protein can be confirmed by western blot, as shown for these frameshift knockout cell lines (α).


Deletions are an alternative method to disable gene function, particularly useful for where the small insertion or deletion that is produced by a frameshift is usually not sufficient to block function.

CRISPR cell line generation applications

Horizon now offers the generation of cell lines in which larger defined regions are excised by CRISPR-Cas9, combining two guide RNAs and screening for clones that lack the intervening DNA sequence to ensure that the gene is disrupted.

This is particularly useful for non-coding parts of the genome:

  • Promoters
  • Enhancers
  • MicroRNA



Point Mutation Cell Lines

Disease relevant mutations can be recapitulated or even correct by introducing a point mutation with a single base change, or by inserting/deleting large gene sequences. These endogenous knockin cell lines enable a clear understanding of the contribution of the gene or mutation to a phenotype, in contrast to overexpression models where high expression (either transiently or stably) can lead to false positives. Horizon produces rAAV and CRISPR knockin cell lines or a combination of both.

Making Knockin Cell Lines

how_we_make_knockin_diagram_1.pngA double-strand break is introduced by targeting the CRISPR-Cas9 to a specific site in the genome, in the presence of a suitably designed homologous donor sequence. If the repair of that double strand break occurs via the homology-directed repair pathway, the donor sequence can be inserted and a single base change is introduced in to that gene.





Reporter Cell Lines

Horizon and Promega Corporation have partnered in a long-term relationship to develop a suite of best-in-class endogenous pathway tag and reporter cell lines for application in functional genomics research and drug discovery and development. Gene-editing at the endogenous level allows researchers to examine near-physiological levels of protein and promoter activity. Traditional methods of understanding protein/promoter function have used exogenous plasmid-based over-expression systems which can result in artefactual results.

Below are some of the reporter cell line application that the team at Horizon Discovery are experienced at working with:

Turbo-GFP

Award winning tagged isogenic cell line pairs for applications where rapid appearance of bright fluorescence is crucial, such as cell and organelle labelling or tracking promoter activity.

Fluorescent Organelles

Horizon's ready-made tagged organelle cell lines enable real time tracking of protein activity in live cells. These are a great alternative to unreliable antibodies.

NanoLuc

The brightness of NanoLuc combined with its small size, high thermal stability, activity over a broad pH range, lack of post-translational modifications and uniform cellular distribution make it an ideal tool for detection of endogenous gene transcription and protein expression.

HaloTag

The HaloTag ligands are designed to covalently bind the HaloTag protein and come in a variety of types including fluorescent ligands and solid substrates, enabling researchers to use HaloTag in a wide variety of applications such as live cell imaging and isolation of HaloTag and therefore the protein of interest.

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