On this page:

• BioXP 3250
• Workflows
• mRNA synthesis products
• Gene synthesis products
• DNA cloning products
• DNA amplification products
• Libraries

BioXp™ 3250 system

The BioXp™ 3250 system provides hands-free, end-to-end automation of DNA assembly, cloning, amplification, and mRNA synthesis in a single overnight run

The award-winning  BioXp™ 3250 system accelerates the design-build-test phases of the product development cycle by enabling automated, rapid, high-throughput synthesis of genes, clones, variant libraries and mRNA. The BioXp™ system is designed to improve productivity, reduce turnaround time, increase throughput and scale, enhance quality, and enable complete workflow control for both synthetic DNA and mRNA formats.

• Eliminates critical bottlenecks + accelerates workflows from weeks and months to days and hours
• First + only push-button system for on-demand biologics printing (DNA, RNA, proteins)
• Unprecedented quality + performance category winner in speed, fidelity, complexity (AI), build size, and scale
• 20–50x productivity increase during design, build phases, resulting in accelerated product development cycles
• Potential to enable global distributed manufacturing for mRNA therapeutics, vaccines, and biologics

Workflows

mRNA-based Therapeutics An mRNA-based approach to therapeutics has recently emerged as an alternative to monoclonal antibody-based drugs	Genome Editing High-throughput design for CRISPR experiments	Genome Synthesis Engineer large, complex, fully-synthetic genomes
Metabolic engineering Reconstruct and optimise biosynthetic pathways with speed and accuracy	Antibody Drug Discovery Advance discovery by accelerating screening and optimisation	Immune Monitoring Rapid screening for safe, responsive immunotherapy treatment
Synthetic mRNA Accelerating discovery by automating mRNA synthesis	Vaccine Development & Discovery Accelerate development and improve efficiency	Precision Medicine Increase throughput. Eliminate contaminants and immunogenicity.

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Products

mRNA synthesis - Automate and accelerate DNA sequence to mRNA

BioXp™ small-scale mRNA synthesis kit 
Contains all the Gibson Assembly® reagents necessary to synthesize up to ten micrograms of biologically active synthetic mRNA using de novo synthesized, error-corrected gene fragments (mRNA template) of up to 1.8 kb in length. 
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Product Specifications:

• Size:  0.3–1.8 kb
• Yield (length- and sequence-dependent): 1–10 µg
• Features:  5' ARCA cap; poly(A) tail (median: 150 As)
• Throughput:  8 or 16 samples per run
• Classification:  RUO reagents

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Gene Synthesi s - Any size. On demand. In your lab. 

BioXp™ gene synthesis kit
BioXp™ gene synthesis kits contain all of the Gibson Assembly® reagents necessary to make error-corrected, de novo synthetic gene fragments of up to 1.8 kb in length. 
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Product specifications:

• Size: 0.3–1.8 kb
• Vector options: Codex DNA vector or your own
• Price: Starting at 8¢ per bp
• Minimum order: 1 gene
• Yield: 100 ng–15 μg
• System runtime: < 18 hours
• Total turnaround time: 3–5 days from order to cloned genes

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DNA cloning - Build complex DNA constructs overnight, on your benchtop

BioXp™ DNA cloning kits
BioXp™ DNA cloning kits contains all of the Gibson Assembly® reagents necessary to make error-corrected, de novo synthetic genes of up to 7 kb in length. These can use either the standard made-to-stock vectors or your specific custom vector. 
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Product specifications

• Size: 300 to 7,000 base pairs
• Yield: 200 ng to 10 μg
• System runtime: 6 to 21 hours; variable, based on application
• Total turnaround time: 3 to 5 days from order to cloned genes

For laboratory scientists performing high-throughput applications that require a rapid turnaround time of error-free clones, the BioXp™ system is the ideal platform for rapidly building high-fidelity constructs. The BioXp™ workflow enables scientists to generate guaranteed error-free clones in under one week, resulting in greater productivity.

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DNA Amplification - Build and amplify complex DNA constructs quickly

BioXp™ RapidAMP™ cell-free DNA amplification kit

BioXp™ RapidAMP™ cell-free DNA amplification kits contain all of the Gibson Assembly® reagents necessary to amplify error-corrected genes, cloned into either a made-to-stock or customer vector, to make up to ten micrograms of DNA.
Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Cloning without E. coli — get to your results faster. Get to your results faster and in fewer steps with Gibson Assembly® RapidAMP™ isothermal amplification. Assemble and amplify transfection-ready DNA in a simple two-day workflow. Achieve over 10 µg of high-quality, high-fidelity DNA with RapidAMP™ technology, and skip the tedious tasks associated with transformation, cell culture, and E. coli harvest.

Product Specifications:

• Max # of fragments assembled:  4
• Cloning efficiency:  95% full-length clones
• Insert size range:  300–1,800 bp
• Total construct size range:  10,000 bp
• Reaction time:  80 minutes + 4 hours

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Build DNA variant libraries - Synthetic DNA, simplified

BioXp™ libraries kits are available with varied single contiguous amino acid sites, including site-saturation such as NNN, NNK, or any other IUPAC nucleotide code, varied single contiguous alanine sites, or varied, multiple non-contiguous amino acids sites using degenerate bases to optimize protein binding and function. Through the power of the BioXp™ system and application-specific BioXp™ kits, Codex DNA enables push-button, walk-away, end-to-end automation of important synthetic biology workflows.

Scanning libraries

BioXp™ site saturation scanning libraries kits and BioXp™ alanine scanning libraries kits provide a wide variety of scanning libraries with varied single, contiguous amino acid sites, including site-saturation (NNN, NNK) and alanine scanning libraries. Design and build your own custom libraries or let our expert team guide you.

Product Specifications:

• Product format:  Linear double-stranded DNA
• Output:  96-well plate; 32 variants per plate; 1 position per well
• Yield:  200 ng–1 µg per position (length-dependent)
• Minimum order:  32 variants
• System runtime:  from 6-7 hours
• Total turnaround time:  3-5 days from order to built libraries

Combinatorial libraries

BioXp™ combinatorial libraries kits provide a wide variety of combinatorial libraries with varied, multiple non-contiguous amino acids sites using degenerate bases to optimize protein binding and function. Design and build your own custom libraries or let our expert team guide you.

Product Specifications:

• Product format:  Linear double-stranded DNA
• Output:  96-well plate; 32 variants per plate; 1 library per well
• Yield:  200 ng–1 μg per position (length-dependent)
• System runtime:  6–7 hours
• Total turnaround time:  3–5 days from order to built libraries

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