Human Immune Monitoring

Introducing a groundbreaking approach to comprehensive human immune monitoring

Whether researchers are following the immune response to treatments for cancer, infection or autoimmune disease, or searching for biomarkers of disease onset and progression, they need a comprehensive, reliable method to monitor changes in immune cell populations.

fluidigm-new-logo.jpgMass cytometry has proven to be a uniquely empowering platform for immune profiling and biomarker discovery. Now, Fluidigm makes this powerful technology more accessible than ever with the Maxpar® Human Immune Monitoring Panel Kit designed for use on Helios™, a CyTOF® system.

Highlights

  • Comprehensive - Identify and deeply profile all major leukocyte populations in human PBMC.
  • Powerful - Maximise valuable information from limited or precious samples.
  • Reliable - Obtain trusted results with the pre-optimised mass cytometry panel.

The Maxpar Human Immune Monitoring Panel Kit

This 29-marker panel kit maximizes the information obtained from every drop of precious sample, enabling identification of over 25 key immune cell populations, all in a single tube (Figure 1).

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Figure 1. Cell subsets that can be identified with the single-tube Maxpar Human Immune Monitoring Panel Kit.

Panel Kit Contents and Workflow

Whether you are new to mass cytometry or are a seasoned pro, the Maxpar Human Immune Monitoring Panel Kit provides the most convenient and efficient approach to identifying all major immune subsets in peripheral blood mononuclear cells (PBMC).

This kit contains all reagents needed to prepare PBMC samples for mass cytometric analysis on Helios, plus:

  • 29 individually packaged, pre-titrated antibodies ready to use straight from the kit to prepare the antibody cocktail
  • A detailed, kit-specific protocol for sample preparation, instrument setup and sample collection on Helios systems
  • A Helios acquisition template pre-populated with the appropriate parameter names and metals required

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Table 1. The 29 anti-human antibodies included in the Maxpar Human Immune Monitoring Panel Kit. The eight open channels are 145Nd, 150Nd, 156Gd, 159Tb, 161Dy, 170Er, 172Yb, 209Bi.

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Figure 2. Workflow for the Maxpar Human Immune Monitoring Panel Kit. No panel design or antibody titrations need to be performed. Stain PBMC samples according to the custom protocol provided, collect data on a Helios or CyTOF 2-to-Helios instrument using the customized kit acquisition template, and analyze samples.

Data analysis options are flexible

FCS 3.0 data files are generated when acquiring samples with the Maxpar Human Immune Monitoring Panel Kit on Helios and can be analyzed by any capable third-party software tool. Manual gating can be performed with third-party software tools such as Cytobank.

Automated analysis software such as GemStone™ can also be used, providing the following features:

  • Automated gating of viable single cells
  • 2D dot plot and cell stage progression profiles
  • High-dimensional Cen-se'™ map of identified populations
  • Sample report for each population, with cell number, percent of total cells and percent of parent population

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