Introducing Microbial Multiplexing Kits for the Sequel System

pacbio_shortlogo_color_rgb-01.jpgWe are pleased to announce two new validated barcoded adapter kits optimized for de novo microbial multiplexing on your Sequel System. These validated barcodes are recommended for high-throughput microbial genome multiplexing experiments, particularly when pooled genomes share high homology. Each kit offers 8 barcoded adapters (6 reactions each) to multiplex up to 30 Mb microbial genomes on a single SMRT Cell, and up to 40 Mb with high-quality genomic DNA.

New Consumable Part Numbers:

  • Barcoded Adapter Kit 8A: 101-081-300
  • Barcoded Adapter Kit 8B: 101-081-400


The new workflow leverages the latest advancements with Sequel Binding/Sequencing Kit 2.1 and SMRT Link v5.1.0. Key benefits include cost reduction through multiplexing, faster time to results, and advanced genome assembly parameters optimized for microbial genomes.

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Below is the new microbial multiplexing protocol and supporting resources from PacBio to help get you up and running with the new workflow:


This Application Note details the considerations needed to achieve cost-efficiency with example case studies. Pacbio have also released the original sequencing dataset and correspondingly assembled genomes from the application note.

For more information please contact our sales team.

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