NGS Library Preparation in SARS-CoV-2 Research

rolo_cmyk.jpg As you seek the best path to advance your research during this pandemic, we at Roche want to help you wherever we can. In addition to distributing millions of diagnostic tests for the detection of SARS-CoV-2 RNA virus or of high-affinity antibodies against that virus, we at Roche want to enable your research into this deadly virus through next-generation sequencing (NGS).

Below, we share some examples of how labs like yours have employed our Research Use Only solutions for NGS library preparation in SARS-CoV-2 research. The publications below describe recent advances in our understanding of COVID-19 pathogenesis and pathophysiology:


screenshot_2020-08-28-dnaimage3-99028a079e01453c-bmp-bmp-image-162-170-pixels.pngPatient-derived mutations impact pathogenicity of SARS-CoV-2. Yao, H. et al. 2020

Yao et al. performed whole viral genome sequencing using KAPA RNA HyperPrep kit with COVID-19 patient isolates to understand whether acquired mutations of the SARS-CoV-2 genome have an impact on the pathogenicity of the virus.

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screenshot_2020-08-28-dnaimage3-99028a079e01453c-bmp-bmp-image-162-170-pixels.pngExpression of SARS-CoV-2 Entry Molecules ACE2 and TMPRSS2 in the Gut of Patients with IBD, Burgueno, J.F. et al. 2020 Inflamm Bowel Dis.

Burgueno et al. used the KAPA RNA HyperPrep Kit with RiboErase (HMR) and the LightCycler® 480 System to understand the expression of the viral entry molecules angiotensin I converting enzyme 2 (ACE2) and transmembrane serine protease 2 (TMPRSS2) in the intestine. Additionally, the authors investigated how inflammatory states in the gut of patients suffering from inflammatory bowel disease (IBD) and taking immune-modulating drugs may affect regulation of ACE2 and TMPRSS2 expression and, therefore, affect SARS-CoV-2 infection.

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screenshot_2020-08-28-dnaimage3-99028a079e01453c-bmp-bmp-image-162-170-pixels.pngT ranscriptional profiling of immune and inflammatory responses in the context of SARS-CoV-2 fungal superinfection in a human airway epithelial model. de Lamballerie, C.N. et al. 2020

Following construction of poly(A)-enriched RNA-seq libraries with the KAPA mRNA HyperPrep Kit, de Lamballerie et al. compared the host transcriptional response to SARS-CoV-2 infection alone to that of SARS-CoV-2 with Aspergillus superinfection to elucidate the impact of fungal superinfections on the course and severity of viral infections in respiratory epithelial cells.

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Thank you for continuing to drive research forward, even during this pandemic.
If you feel that Roche's library prep and target enrichment solutions would aid you in your research, please reach out to us directly; we're with you, every step of the way.


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