PacBio - Monthly Newsletter (November)

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Welcome to the PacBio News and Events Update!

Contents:


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The ANZ HiFi SMRT Grant ran from 23 Aug through to 12 Oct, and a total of 74 submissions were received! This is by far a record number of submissions received for a Local APAC SMRT Grant. Stay tuned for more updates as we pick the winning submissions and finalists!


New Sequel II System On-Line in Perth

Recently we installed a Sequel II system for Genomics WA. Located at the Telethon Kids Institute, the system will be open soon for researcher to access and submit their samples. 

Contact us to register your interest in gaining access


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Whether you're an avid PacBio user or someone interested in learning how highly accurate long-read sequencing (HiFi sequencing) can help you answer your toughest biological questions, the PacBio Virtual Global Summit has something for you.

This 2-day event will provide an opportunity to hear from your peers about experiences and results generated on the Sequel and Sequel II Systems throughout a variety of sessions:

"Interactive SMRT Sessions" have limited capacity – take a look now and  save your seat

Register Now

Download the Agenda


ASHG - PacBio Workshop on Demand

ashg-image.jpgIn this online workshop, which is available to watch now on-demand, PacBio Chief Scientific Officer Jonas Korlach introduc es the  new Sequel IIe System  as well as our  latest applications  using highly accurate long reads (HiFi reads), including human WGS for variant detection,  de novo  genome assembly, single-cell RNA sequencing, and targeted sequencing using PCR and No-Amp methods.

Now that  ASHG has wrapped up, there are ton of posters and presentations which highlight how PacBio technology has been used by human biomedical research scientists.  Check them out here .



Sequel IIe Release  

We are pleased to announce the launch of our new instrument, the Sequel IIe System.  

The Sequel IIe System is an evolution of the award-winning Sequel II System with the same high performance and reliability, but now featuring direct access to the most valuable and informative sequencing data: PacBio HiFi reads. With this new system, and the simplicity of HiFi data, you can expect a 90% reduction in file storage needs, and a 70% reduction in secondary analysis processing time, letting you go from sample to biological insights quickly and easily.

Download Announcement 

Download Product Brochure


SMRT Link v10.0 Software Package Release  

Available for download in November 2020. 

SMRT Link v10.0 provides powerful new tools, including complete workflow integration on the AWS Cloud and a new Genome Assembly analysis application for generating reference-quality de novo assemblies from HiFi reads.


New SMRTbell gDNA Sample Amplification Kit  

Explore a preprint showing high quality de novo genomes from single specimens of field-collected and ethanol-preserved springtails

The new SMRTbell gDNA Sample Amplification Kit used for this project enables whole genome amplification starting from as little as 5 ng of gDNA, allowing you to use samples such as insects/arthropods and limited human DNA samples (e.g. needle biopsies). 

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The SMRTbell gDNA Sample Amplification Kit is designed to amplify samples starting from ~5-20 ng of gDNA to a sufficient quantity for template preparation procedures using the SMRTbell Express Template Prep Kit 2.0. The kit contains sufficient reagents to prepare up to 18 samples.

 Application Note:  Considerations for Using the Low and Ultra-low DNA Input Workflows for Whole Genome Sequencing 
Protocol:  Procedure & Checklist - Preparing HiFi SMRTbell Libraries from Ultra-Low DNA Input  
Blog:Now Available: Ultra-Low DNA Input Workflow for SMRT Sequencing


Invitae, Pacific Biosciences Collaboration on epilepsy diagnostic

Invitae and Pacific Biosciences on Thursday announced a research collaboration that will use long-read next-generation sequencing to help develop diagnostic testing for epilepsy.

Check out the joint press release, GenomeWeb article and PacBio blog post  


Pacific Biosciences, Children's Mercy, Microsoft Collaborate on Rare Genetic Disease Diagnostics

genomicanswersforkids.jpgPacBio will collaborate with Children's Mercy Kansas City as part of its Genomic Answers for Kids (GA4K) program, which aims to collect genomic data and health information for 30,000 children and their families over the next seven years, ultimately creating a database of nearly 100,000 genomes

Read the PacBio blogpress release and GenomeWeb article 


Research Insight: Targeting the entire BRCA1 and BRCA2 loci (~200kb)

A new publication entitled "CRISPR–Cas9/long-read sequencing approach to identify cryptic mutations in BRCA1 and other tumour suppressor genes" shows how CRISPR-Cas9 can be used with Sage Science's HLS-CATCH system and PacBio sequencing to fully characterise complete (~200kb) BRCA1 and BRCA2 genomic regions.

The researchers analyzed lymphoblast cells of patients with breast cancer and, in one family, identified an intronic SINE-VNTR-Alu retrotransposon insertion that led to the creation of a pseudoexon in the BRCA1 message and introduced a premature truncation.

Read Full Article 


Research Insight: Advice for metagenomics assemblies

Did you know raw read accuracy has a large impact on metagenome-assembled genome (MAG) completeness, contiguity, and contamination? Read our blog to learn why HiFi reads are so powerful for metagenomics, then view a poster on BVAB1 for a real-world example. Explore our GitHub repository to plan your next experiment! 


Subscribe to the PacBio Monthly Newsletter by Millennium Science here

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