10x Genomics - Monthly Newsletter (February 2021)

10x_logo_vertical_full-color_digital.pngWelcome to our monthly 10x Genomics update!


Contents:


CellPlex Pre-Orders

Scale your single cell studies with our new sample multiplexing kit to get more samples, more cells, and more insights for your single cell gene expression experiments.

CellPlex is now available for pre-order.

End-to-end Sample Multiplexing Workflow

Full support from protocols to pipelines and visualisation

70b60bd2d6a72cadd0deaff40b0c4fe9.jpg

CellPlex Resources:

Contact us to pre-order CellPlex



86fae42f4f3b9bf9333350b7c140b66f.jpg

Xperience 2021

Virtual Event 3rd March

This virtual event will showcase the 10x Genomics 2021 product roadmap and initial data. Q&A sessions will focus on our Chromium single cell platform, Visium spatial platform and In Situ platform.

Register Now



7c913bbf0ba647b91ceee6a743630fdf.jpg

Neuroscience Challenge

For both new and existing researchers to win a chance for multiomics analysis (more than 2 applications).

Submit Application



Fellowships

10x Fellowship applications were invited in late 2020 and we received an overwhelming response with over 50 applications! We are now contacting all successful applicants and organising support events. Thanks to everyone who applied for the program and please look out for the next opportunity!

Register your interest for the next round of the Fellowship program (being considered for late 2021)

Register Now



Visium Support for New Users

Millennium Science is currently working to set up Visium reference sites for new users of spatial transcriptomics.

Contact us if you would like to begin using Visium but need help with where to start.

Contact us about Visium



Frequently Asked Questions

paul.jpg Hello and welcome to the 10x Newsletter.
My name is Paul Gooding and I'm the Field Application Scientist with Millennium Science for their Genomics portfolios in Australia and New Zealand.
I thought it would be fun to host an FAQ section where I could post the most common questions that I get asked about 10x Genomics solutions and technologies, and the most up to date responses. There is also a link to send in your own question, I will do my best to answer it in the following newsletter

Are there gene expression differences between fresh and frozen-thawed cells?  

From in-house tests at 10x Genomics, very little difference was detected. Fresh and frozen-thawed human PBMCs from the same individual were compared using the Single Cell 3' assay. A high correlation of mean UMI counts per gene across all cells between fresh and frozen-thawed conditions was found (r= 0.96).

The median number of UMIs and number of genes detected between the two conditions was also not significantly different (P-value = 0.1 and 0.8, respectively).

Fifty-seven genes showed two-fold upregulation in the frozen sample, with ∼50 % being ribosomal protein genes and the rest not enriched in any pathways.

This data is available in Zheng, G., Terry, J., Belgrader, P. et al. Massively parallel digital transcriptional profiling of single cells. Nat Commun 8, 14049 (2017).

https://doi.org/10.1038/ncomms14049

The effect of frozen/thawed cells vs fresh will likely vary for different cell types due to having different sensitivities to freeze/thaw.

Have a question for Paul? Submit your question here


Subscribe to the 10x Monthly Newsletter by Millennium Science here

Enquiry Form

Required fields are marked with*