Horizon Update

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The world of gene editing and modulation has been advancing at a rapid pace. In our current climate where robust technologies are even more relied upon we are seeing continued adoption of CRISPR across all fields of research including COVID-19 research. Researchers at EMBL Rome are currently using CRISPR  to see if they can silence the SARS-CoV-2 Receptor which is used by the virus to enter it's host cell. Read more.

Below you'll find a collection of blogs, product profiles and webinars to help keep you up to date!

Happy reading!




Blog

Common CRISPR Pitfalls and How to Avoid Them

shutterstock_1382943902-1200-x-628.jpgWith rapidly increasing amounts of information, various reagent formats, and numerous applications, where does one start when planning a gene-editing experiment? What do you need to think about when choosing your biological system? What reagents are suitable for your experimental aim and constraints?

Here we discuss some points, that when considered in advance, will make your CRISPR-based gene editing experiments more successful.

Read More




Product Highlight

Edit-R™ CRISPR Guide RNA

Designed to provide the highest likelihood of functional protein knockout, while minimizing off-target effects.

Functional and Specific Targeting for High-Confidence Gene Knockout Results

synthetic-sgrna-u2os-fluorescent-ko.jpgEdit-R predesigned synthetic sgRNAs are chemically synthesized guide RNA for S. pyogenes Cas9 nuclease targeting and creation of Cas9-mediated DNA double-strand breaks in a genomic region of interest.

All Edit-R guides are algorithmically designed to efficiently generate functional protein knockouts, not just create an insertion or deletion.  Additionally, chemical modifications are applied to all Edit-R synthetic guide RNA products to reduce degradation by nucleases and improve overall editing performance.

This unmatched accuracy and efficiency makes Edit-R predesigned synthetic sgRNA an ideal tool for pathway analysis or following up hits from a synthetic sgRNA library screen.

Learn More in the Feature Article


Required Components for an Edit-R CRISPR-Cas9 Gene Editing Experiment Using Synthetic sgRNA

  • A source of Cas9 nuclease, whether in protein, mRNA, or lentiviral form
  • Edit-R synthetic sgRNA designed to target the gene of interest

All predesigned guide RNAs are fully guaranteed to edit the gene they target, ensuring the highest likelihood of a successful knockout experiment.




Webinar

High-Throughput Genome-Wide RNAi Screens: Insights from the Sheffield RNAi Screening Facility

This webinar provides an overview of a high-throughput RNAi screening from the perspective of a dedicated RNAi screening facility.

Presenter, Stephen Brown, PhD, University of Sheffield includes the aspects of building a screening platform, uses of automation, designing robust screens, experimental controls, and hit validation.

James Goldmyer, PhD presents on our synthetic single guide CRISPR libraries and their benefits.

View Webinar


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