Loop Genomics - Get Better Data with Long Reads

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Looking for a sample prep kit that will give you long-read data from short reads?

Millennium Science proudly represents Loop Genomics – sample prep kits for long reads from Illumina Sequencers and no extra hardware.

Each kit comes with all the reagents you need to get from extracted genomic DNA or RNA to a sequencing ready library - except magnetic purification beads (e.g., SPRI).

Loop Genomics saves you time in the lab with a simplified workflow, you can multiplex all your samples from a single tube, saving you hours in the lab.

Loop is also set up as a service lab. It’s as simple as sending your extracted DNA or RNA samples to us and we’ll get the data and a full computational analysis back to you and your team.

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The Technology

The core of Loop's technology is the unique barcodes which attach to individual DNA molecules. Here’s how it works.

Attach
Every sample is exposed to millions of unique barcodes, but only one barcode attaches per strand of DNA at the 16S site.

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Amplify
Every molecule, along with its unique barcode, is amplified using PCR.

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Distribute
For each molecule copy, the barcode is randomly distributed within the molecule.

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Sequence
Sequence the segment next to each barcode.

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Assemble
Short reads that share the same barcode are combined algorithmically into a full-length molecule using linked-read de novo assembly.

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Benefits:

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Multiplex Workflow
Pool all samples into a single tube in the first step.

Long-Reads
Read V1-V9.
Read 2.5 Kb from 18S through ITS1 & ITS2.

Species Classification
Higher resolution microbiome classification.

True Quantification
Barcode based quantification with no PCR bias.

Contact Millennium Science today to learn more about Loop Genomics!

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