Western Blots

The western blot protocol was introduced in the 1970s as a method of protein detection and is now a routine and ubiquitous technique for protein analysis and a lot of basic biological research. The specificity and high affinity of antibody-antigen interaction enables a target protein to be identified in a complex protein sample. Western blotting can produce qualitative, semi-quantitative and now quantitative data about a protein of interest depending upon which detection method is used. More recent approaches to Western blotting enable multiplex western blot analysis in which multiple proteins can be simultaneously detected and differentiated on a single blot allowing much improved reproducibility and data quality.

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