Products
Sapphire700™ Stain
Sapphire700 Stain is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of A431 cells in 96-well plates, Sapphire700 Stain displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well.
CellTag™ 700 Stain
The In-Cell Western Assay is an immunocytochemical assay that uses near-infrared fluorescence to detect and quantify proteins in fixed cells. Detecting proteins in their cellular context increases quantification precision. Proteins in fixed, cultured cells are detected directly in microplates, which yields higher throughput compared to Western blotting, and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. By using the CellTag 700 Stain ICW Kits, the cost per well is reduced to a fraction of the cost of typical screening methods.
CellVue fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes.1 They are useful for researchers working in all aspects of science and technology where fluorescently labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.
The CellVue NIR815 cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue NIR815) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of staining is dependent upon the cell type being labeled and the membranes of the cells.
PSVue 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria and other anionic membranes. The PSVue 794 reagent kit contains components to provide a 1 mM solution of PSVue 794 in aqueous solution. The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it has been found to bind strongly to negatively charged bacterial cell walls (e.g. S. aureus, E. coli) and necrotic regions present in various tumors (e.g. mammary, prostate, glioma) in vitro and in vivo.
Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has recently emerged as a convenient, versatile, and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development. Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines.
VRDye™ Protein Labeling Kits label antibodies and other proteins for applications such as flow cytometry, microscopy, immunohistochemistry, and other applications where fluorophore-conjugated antibodies are required. VRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. The High MW kits are optimized for labeling 1 mg of an IgG antibody. The High MW-Microscale kits are optimized for labeling 100 µg of an IgG antibody. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.
When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. 
Make Western blot normalization more accurate and reliable with REVERT Total Protein Stain. This quick total protein normalization method detects all sample proteins after transfer from gel to membrane. The stain provides linear signal over a broad range of sample concentrations and is compatible with subsequent Western blot immunodetection methods.
LI-COR® Odyssey Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey Imaging Systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes and with either Odyssey Blocking Buffer (PBS) or Odyssey Blocking Buffer (TBS).
The Quick Western Kit — IRDye® 680RD provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step. This reduces the overall time to complete a Western blot and provides the advantages of near infrared fluorescence detection. The kit also serves as a detection solution for post-immunoprecipitation samples by Western blot because it does not bind to denatured mouse monoclonal or rabbit monoclonal antibodies. The key benefit is the ability to use the same antibody for immunoprecipitation and post-immunoprecipitation detection by Western blot.
The Chemi-IR Detection Kit converts chemiluminescent Western blots to near-infrared fluorescent Western blot detection. Incubation of Western blots with IRDye® 800 Rabbit anti-HRP enables detection of HRP conjugated antibody before or after chemiluminescent detection.
The WesternSure Pen is used to annotate visible protein ladders prior to chemiluminescent Western blot detection. The pen is optimized for detection using the 
Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
Substrate selection is a key component in developing ELISAs with high signal and low background. LI-COR offers proprietary HRP 680 and AP 680 Substrates that are optimized for use in the near-infrared region (680 nm) on the Aerius, and Odyssey® CLx, Odyssey Classic, and Odyssey Sa Infrared Imaging Systems. Near-infrared (NIR) detection overcomes the limitations of chromogenic substrate detection, which does not allow for quantification of greater than four optical density units. NIR detection allows for a wider linear range.