Products
Sapphire700 Stain is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of A431 cells in 96-well plates, Sapphire700 Stain displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well.
The In-Cell Western Assay is an immunocytochemical assay that uses near-infrared fluorescence to detect and quantify proteins in fixed cells. Detecting proteins in their cellular context increases quantification precision. Proteins in fixed, cultured cells are detected directly in microplates, which yields higher throughput compared to Western blotting, and eliminates typical Western blotting steps such as cell lysate preparation, electrophoresis, and membrane transfer. By using the CellTag 700 Stain ICW Kits, the cost per well is reduced to a fraction of the cost of typical screening methods.
Products
Near-infrared fluorescent probes for targeting of tumors, bone, lymphatics, and more.
Benefits
- Validated with cell-based assays, microscopy, small animal imaging, and histology to ensure high affinity and specificity.
- Simply administer the agent, then image the animal with a small animal imager quipped with an appropriate 680nm and 800nm filter set.
- Compatible with most small animal imaging systems.
- Sensitive detection in the animal - even for small or deep targets.
- Ready-to-use simplicity.
- Proven track record of peer-reviewed publications.
CellVue fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes.1 They are useful for researchers working in all aspects of science and technology where fluorescently labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.
Products
The CellVue Burgundy cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue Burgundy) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency.
The CellVue NIR815 cell linker kit uses proprietary membrane labeling technology to stably incorporate a fluorescent dye with long aliphatic tails (CellVue NIR815) into lipid regions of the cell membrane. The labeling vehicle provided with the kit (Diluent C) is an iso-osmotic aqueous solution which contains no physiologic salts or buffers, detergents, or organic solvents and is designed to maintain cell viability while maximizing dye solubility and staining efficiency. The pattern of staining is dependent upon the cell type being labeled and the membranes of the cells.
PSVue 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria and other anionic membranes. The PSVue 794 reagent kit contains components to provide a 1 mM solution of PSVue 794 in aqueous solution. The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it has been found to bind strongly to negatively charged bacterial cell walls (e.g. S. aureus, E. coli) and necrotic regions present in various tumors (e.g. mammary, prostate, glioma) in vitro and in vivo.
Dye contamination found on the surface of animals or within the field of view of the imaging bed will interfere with detection of targets within an animal. External dye contamination can arise from urine residue on the animal's skin or the imaging bed surface. Water and alcohol are not very effective for dye residue removal, and depilatory creams can irritate the skin of the animal. This decontamination kit effectively and safely removes dye contamination from the skin of the animal or surface of Pearl® imaging beds.
Products
IRDye infrared fluorescent dyes and near-infrared (NIR) fluorescence imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios. IRDye fluorescent dyes have absorption and emission wavelengths in the NIR spectrum, between 680 and 800 nm. Most of the IRDye reagents are compatible with the Odyssey® CLx, Odyssey Fc, Odyssey Sa, Pearl® Imagers, and 4300 DNA Analyzer platforms.
Features
- Low background autofluorescence allows higher signal to noise ratios.
- Wide range of dyes and functional groups available for multiple applications.
- Available in custom derivations of fluorescent dyes and alternative pack sizes through Custom Labeling and Synthesis Services.
- Excellent photostability
- Dye Brightness
IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In‑Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services.
Biomolecule labeling continues to be a cornerstone feature of many in vitro and in vivo biological experiments. Click Chemistry has recently emerged as a convenient, versatile, and reliable method for labeling a wide variety of molecules for applications ranging from biomarker isolation to assay development. Click Chemistry utilizes pairs of reagents that exclusively react with each other and are effectively inert to naturally occurring functional groups such as amines.
Features
- Versatile alternative to affinity-based detection.
- Chemoselective, easy-to-perform, high yielding reactions.
- Offer flexibility with a choice for copper-catalysed and copper-free reactions.
VRDye™ Protein Labeling Kits label antibodies and other proteins for applications such as flow cytometry, microscopy, immunohistochemistry, and other applications where fluorophore-conjugated antibodies are required. VRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services. The High MW kits are optimized for labeling 1 mg of an IgG antibody. The High MW-Microscale kits are optimized for labeling 100 µg of an IgG antibody. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.
Products
When performing two-color detection, loading controls and normalization are essential for reliable, precise comparison of protein levels. LI-COR offers five primary antibodies for normalization — antibodies to actin, tubulin, and COX IV — that can be used for two-color normalization when performing multiplex Western blots or for cell-based assay normalization, such as for In-Cell Western™ Assays.
Features
- Normalise and correct for uneven loading, using the intensity of the internal control band.
- Visually compare protein levels with confidence, even if you don't quantify bands.
- Ensure that visually-observed changes in protein levels represent actual change, not artifacts.
Make Western blot normalization more accurate and reliable with REVERT Total Protein Stain. This quick total protein normalization method detects all sample proteins after transfer from gel to membrane. The stain provides linear signal over a broad range of sample concentrations and is compatible with subsequent Western blot immunodetection methods.
Features
- Quick and Compatible. Stain total protein in less than ten minutes on either PVDF or nitrocellulose membranes.
- Accurate Nomalisation. With a wide linear range of 1-60 µg, it's easy to detect REVERT and your target in the same linear range for accurate normalisation.
- Reliable Analysis. Unlike housekeeping proteins, biological variation won't affect total protein normalisation with REVERT.
Products
LI-COR® Odyssey Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey Imaging Systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes and with either Odyssey Blocking Buffer (PBS) or Odyssey Blocking Buffer (TBS).
Applications
- Quantitative Western Blots.
- In-Gel Westerns.
- RNAi Studies.
The Quick Western Kit — IRDye® 680RD provides a universal detection reagent that can be combined with the primary antibody incubation step, eliminating the need for a secondary antibody incubation step. This reduces the overall time to complete a Western blot and provides the advantages of near infrared fluorescence detection. The kit also serves as a detection solution for post-immunoprecipitation samples by Western blot because it does not bind to denatured mouse monoclonal or rabbit monoclonal antibodies. The key benefit is the ability to use the same antibody for immunoprecipitation and post-immunoprecipitation detection by Western blot.
Benefits
- Does not require a separate primary antibody labeling step, saving time and antibody.
- Faster method of detection compared to the traditional 2-step method, which can take up to 4 hours.
- Reduces the total Western blot procedure by at least 90 minutes.
- Eliminates the use of labeled secondary antibodies and associated washes.
- Eliminates the need to purchase multiple secondary antibodies.
- Use the same antibody for immunoprecipitation and post-IP detection by Western blot.
- Works with a variety of primary antibodies.
- Has been shown to recognise primary antibodies to recombinant tagged proteins.
The Chemi-IR Detection Kit converts chemiluminescent Western blots to near-infrared fluorescent Western blot detection. Incubation of Western blots with IRDye® 800 Rabbit anti-HRP enables detection of HRP conjugated antibody before or after chemiluminescent detection.
Benefits
- Use your existing HRP secondary antibodies.
- Detect on Odyssey Infrared Imaging Systems.
- Eliminate substrates, film, and the darkroom.
- Make familiar chemiluminescent methods quantitative.
- Optimise your Western blot results.
Products
Gel shift assays, or electrophoretic mobility shift assays (EMSA), provide a simple method to study DNA-protein interactions. Existing mobility shift assay protocols can be easily converted to infrared fluorescent assays by replacing the existing DNA oligonucleotides with IRDye® infrared dye end-labeled oligonucleotides. Binding and electrophoresis conditions are the same as any other EMSA detection method.
Substrate selection is a key component in developing ELISAs with high signal and low background. LI-COR offers proprietary HRP 680 and AP 680 Substrates that are optimized for use in the near-infrared region (680 nm) on the Aerius, and Odyssey® CLx, Odyssey Classic, and Odyssey Sa Infrared Imaging Systems. Near-infrared (NIR) detection overcomes the limitations of chromogenic substrate detection, which does not allow for quantification of greater than four optical density units. NIR detection allows for a wider linear range.