Reveal the Full Complexity of Cellular Diversity, Cell by Cell
Chromium Single Cell Gene Expression provides single cell transcriptome 3' gene expression and multiomic capabilities to profile tens of thousands of cells. Explore cellular heterogeneity, novel targets, and biomarkers with combined gene expression, surface protein expression, or CRISPR edits in each cell.
Features:
- Single Cell Resolution at Scale - Capture the full heterogeneity of a sample, in tens of thousands of cells.
- Multiomic Cellular Readout - Measure 3' mRNA, cell surface proteins, CRISPR perturbations, and more.
- Scalable Functional Genomics - Explore gene networks with paired CRISPR knockdowns and whole transcriptome readouts.
- Streamlined Data Analysis - Explore and interpret single cell gene expression profiles with easy-to-use software.
- One-day Lab Workflow - From sample to sequencing-ready library in one day.
- Diverse Sample Compatibility - Demonstrated on fixed, fresh, and frozen samples, as well as whole cells and nuclei.
Capabilities for multiomic readouts
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Cell Surface Protein
Explore combined single cell gene and protein expression to enable discovery of novel cell subsets and rare cell populations.
- Enrich distinct cell phenotype understanding with added protein information
- Explore transcriptome and proteome relationship with simultaneous single cell detection
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CRISPR Screening
Assess effect for CRISPR perturbations at single cell resolution with direct capture of cellular sgRNAs and changes in gene expression.
- Screen for novel gene targets of resistance drivers involved in disease
- Empower functional genetic screens with a faster workflow and increased resolution
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Unravel the complex genetic regulatory pathways involved in development
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The base workflow for Single Cell Gene Expression is shown below. If you're using the Feature Barcoding Kit for CRISPR or Cell Surface Protein, 10x provides additional protocols for modifying your cells of interest.
Step 1: Prepare Your Sample
Start with hundreds to thousands of cells. Follow best practices for washing, counting, and concentrating cells to minimize the presence of cellular aggregates to retrieve high quality single cell suspensions. This minimizes the presence of cellular aggregates and debris.
Step 2: Construct Your 10x Library
Construct a 10x barcoded library using our reagent kits and Chromium Controller. The Chromium Controller encapsulates each cell with a 10x barcoded Gel Bead in a single partition. Within each nanoliter-scale partition, cells undergo reverse transcription to generate cDNA, where all cDNA from individual cells share a common 10x barcode.
Step 3: Sequence
The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers, for massive transcriptional profiling of thousands of individual cells.
Step 4: Analyse Your Data
Our Cell Ranger analysis software generates expression profiles for each cell, identifies clusters of cells with similar profiles, and aggregates data from multiple samples.
Step 5: Visualise Your Data
Use our Loupe Browser visualization software to interactively explore your results, study expression patterns for genes of interest, determine cell types, and perform comparative analysis between clusters and samples.
