Empiria Studio Software

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Expert Analysis Made Simple

Empiria Studio Software is a post-processing, Data Integrity Software for quantitative data analysis. Created in partnership with high impact journals, Empiria Studio provides key advantages over signal identification software—such as step-by-step workflows, validation and analysis features, assay development tools, and shareable files for easy collaboration.

Outperform with Data Integrity Software

A signal identification software only identifies an image's signal levels. As the only available Data Integrity Software, Empiria Studio quantifies signal levels using raw pixel intensity and provides expert data analysis. It incorporates publishers' best practices and systematic workflows for faster, consistent, and more reliable results among all users. When compared to a signal identification software, Empiria Studio users achieve a significantly lower % CV and complete their analyses in less time.

Step-By-Step Workflows

Empiria Studio's workflows are based on industry best practices and designed to walk lab members of all experience levels through the validation, analysis, replicability, and publication stages of quantitative data analysis. This step-by-step process ensures consistency in and the reliability of each researcher's results. In particular, they also help develop and optimize In-Cell Western™ Assays with key steps—such as antibody titration, blocker evaluation, cell stain linearity, and target analysis.

With the Empiria Studio Experiment Designer, you can even design an experimental microplate map in Empiria Studio. This feature helps chart your microplates well-by-well, so you know which ones contain positive and negative controls, background, and treatments. Together, the workflows and a new Template Library and Emperiment Designer make setup and data analysis easier than ever before.

Validation Features

Antibody Validation 

Confirm that the primary antibody is specific and selective to the target.

Linear Range Validation 

Determine sample loading that will fall within a combined linear range of the target and internal loading control.

Housekeeping Protein (HKP) Validation

Verify that the selected HKP is stably expressed and not affected by experimental conditions before using it as an internal loading control.

Analysis and Publication Features


Quantify the internal loading control and targets, allowing Empiria Studio to automatically normalize the target signals to the internal loading control.

Adaptive Lane Finding

Automatically detect lane number and boundaries for faster analysis, fewer sources of variability, and improved total protein stain quantification.

Adaptive Background Subtraction

Automatically detect and subtract image background for minimal or eliminated user bias and impact from subjective choices.

Replicate Analysis

Compare replicate target analyses to confirm the accuracy of observed changes and get statistically significant results.

Publication Packs

Create and easily share packages containing experimental data and procedures with publishers and colleagues.

Empiria Studio and Odyssey Imagers

Empiria Studio is designed to integrate seamlessly with Odyssey Imagers, making it easy to import raw images and generate publishable results via the step-by-step workflows. With standardized methods and validation, analysis, assay development, and replicability features, each Odyssey Imager user can perform analyses correctly and consistently—regardless of their level of experience.


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