Map the Whole Transcriptome Within the Tissue Context
Visium Spatial Gene Expression is a next-generation molecular technology for classifying tissue based on total mRNA. Map the whole transcriptome with morphological context to discover novel insights into normal development, disease pathology, and clinical translational research.
Features:
- Protein co-detection - Combine whole transcriptome spatial analysis with immunofluorescence protein detection.
- Profile entire tissue sections - No need to select regions of interest - analyse the whole transcriptome from an entire section.
- High resolution - 1-10 cell resolution on average per spot depending on tissue type.
- Streamlined data analysis - Combine histological and gene expression data with easy-to-use software.
- One-day lab workflow - From sample to sequencing-ready library in one day.
- Diverse sample compatibility - Demonstrated on a diverse set of organs across species (human, mouse, rat, and more).
Extendable for Protein Co-detection
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Protein Co-Detection
Enable discovery of novel cell types and their spatial organisation by combining immunofluorescence and whole transcriptome spatial gene expression.
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Explore transcriptome and protein relationships with simultaneous spatial detection
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Discover new tissue biomarkers with cell type specificity
Download Product Sheet
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Step 1: Prepare Your Sample
Embed, section, and place fresh-frozen tissue onto a capture area of the gene expression slide. Each capture area has thousands of barcoded spots, each containing millions of capture oligonucleotides with spatial barcodes unique to that spot.
Step 2: Stain the Image and Tissue
Utilise standard fixation and staining techniques, including Hematoxylin and Eosin (H&E) staining, to visualize tissue sections on slides using a brightfield microscope.
Step 3: Permeabilise Tissue and Construct Library
Permeabilise the tissue to release mRNA from the cells, which binds to the spatially-barcoded oligonucleotides present on the spots. A reverse transcription reaction produces cDNA from the captured mRNA. The barcoded cDNA is then pooled for downstream processing to complete a sequencing-ready library.
Step 4: Sequence
The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers for massive transcriptional profiling of entire tissue sections.
Step 5: Analyse and Visualise Your Data
Use our Space Ranger analysis software to process your Spatial Gene Expression data and interactively explore the results with our Loupe Browser visualization software.
Visium Spatial Gene Expression Solution | Visualize Gene Expression within Tissue Organization
