Spatial Proteomics

Tissue Profiling with Transcriptomics and Protein Co-detection

Combine whole transcriptome spatial analysis with immunofluorescence protein detection in the same tissue section. Gain a deeper, more holistic understanding of tissue organisation with colocalised protein detection and gene expression analysis.

Features:

Maximise your Insights - Gain a deeper, more complete assessment of your samples with protein, total mRNA, and morphology in one.

Profile Entire Tissue Sections - Visualise protein and whole transcriptome expression, without selecting regions of interest.

Accessible Technology - Use standard microscopy, immunofluorescence staining protocols, and antibodies.

Streamlined Data Analysis - Combine histology or protein expression profiles with gene expression data in easy-to-use software.

One-Day Lab Workflow - From tissue section to sequencing-ready library in one day.

Diverse Sample Compatibility - Demonstrated on multiple mouse and human tissues.

Step 1: Prepare Your Sample

Embed, section, and place fresh-frozen tissue onto a capture area of the gene expression slide. Each capture area has thousands of barcoded spots, each containing millions of capture oligonucleotides with spatial barcodes unique to that spot.

Step 2: Stain and Image the Tissue

Step 3: Permeabilise Tissue and Construct Library

Permeabilise the tissue to release mRNA from the cells, which binds to the spatially-barcoded oligonucleotides present on the spots. A reverse transcription reaction produces cDNA from the captured mRNA. The barcoded cDNA is then pooled for downstream processing to complete a sequencing-ready library.

Step 4: Sequence

The resulting 10x Barcoded library is compatible with standard NGS short-read sequencing on Illumina sequencers for massive transcriptional profiling of entire tissue sections.

Step 5: Analyse and Visualise Your Data

Use the Space Ranger analysis software to process your Spatial Gene Expression data and interactively explore the results with the Loupe Browser visualisation software.

Millennium Science

Enabling next-generation research

Tissue Profiling with Transcriptomics and Protein Co-detection

Features:

Workflow

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