The first HiFi benchtop system – bringing industry-leading and exceptionally accurate long reads to your lab.

The Vega benchtop system delivers up to 60 Gb of HiFi data per run, methylation calling included, using a single SMRT® Cell. The onboard compute provides accurate base calling, HiFi read generation with DeepConsensus, methylation calling, barcode demultiplexing, and BAM file generation.

HiFi reads turn questions into answers by offering accurate detection of variant types from SNPs to structural variants with high precision and recall, even in challenging regions of genomes. A subset of key applications include:

Ever feel like you’re being asked to do more research with less funding? We see you! That’s why we’re launching Chromium Xo.

Now you can bring the advantage of superior reproducibility and time savings from automated, instrument-supported single cell partitioning to your bench for a significantly lower cost. Paired with industry-leading GEM-X technology for 3’ whole transcriptome profiling, Chromium Xo lets you single cell with confidence.

As we work to make single cell more accessible at lower costs, Chromium Xo is just the start. New Chromium innovations are coming soon, continuing our commitment to provide superior performance over alternative methods and reduce budget constraints at every step. What does that look like in your lab? Find out below.

Get the instrument-supported Chromium advantage

• Automate the most critical steps for more reproducible results and a lower risk of manual error than with instrument-less methods
• Ensure high success rates and consistent performance, with a demonstrated R² value of 0.97 across runs, users, and timepoints
• Partition tens of thousands of cells in mere minutes, saving on time and labor costs with 1-day workflows and just 3–4 hours of hands-on time
• Use our intuitive push-button interface for efficient cell processing without the hassle of cumbersome pipetting methods

Achieve superior performance for your gene expression studies

Source: Single Cell 3′ v4 (GEM-X) dataset and Parse Evercode v3 dataset¹

• Make the most of every sample with industry-leading GEM-X 3’ assays that provide up to 80% cell capture and >40% higher gene sensitivity than alternative methods
• Capture more accurate biology from a wide range of species with high-quality results for low-expressed transcripts and fragile cells
• Generate more usable reads and detect more genes at lower read depths, saving up to 50% on sequencing costs
• Gain comprehensive views of complex biology with simultaneous capture of protein and gene expression

Read about the performance advantages of Chromium assays over alternative methods in this independent Genentech study comparing single cell platforms >

Move your research forward with built-in convenience and flexibility

• Run experiments as needed, no potential wait times or additional labour costs/fees
• Easily increase the frequency or number of single cell projects as your research needs grow
• Take advantage of fast and easy instrument management—including support and software updates—with 10x Genomics Cloud connectivity
• Upgrade your instrument with ease to access the full breadth of the Chromium portfolio, including assays for FFPE samples and immune profiling

Check out the 10x Genomics instrument family page to explore options for more sample types, additional multiomic readouts, and applications >

Ensure your success with end-to-end support

We’re thrilled to be making single cell more accessible than ever before. New advancements are just ahead, with incredible options to continue to drive down your costs across a wide range of applications. Stay tuned!

• Expertly curated 5,000-gene panels that let you explore your biology with remarkable depth and breadth.
• Outstanding sensitivity, specificity, and accuracy that inspires confidence in each one of your transcripts.
• The fastest way to image thousands of genes in thousands of cells across your entire tissue section.

Explore the Xenium Prime 5K human lymph node dataset

Xenium Prime 5K assays are optimized for performance and paired with our advanced multimodal cell segmentation, enabling exquisite sensitivity, specificity, and spatial fidelity. This means ultra-high-plex data without sacrificing data quality and greater confidence in each transcript assignment.

The Agilent BioTek 406FX Washer Dispenser is a compact instrument offering fast, full plate washing along with up to six reagent dispensers.

It features a Dual-Action manifold that optimises plate washing for a variety of cell types, in addition to bio-magnetic protocols, across a broad range of plate configurations, from 96 to 1536 wells.

Unlike typical microplate dispensers that rely solely on peristaltic or syringe pumps, the 406FX includes up to four syringe pumps and two peristaltic pump dispensers to enable rapid dispensing of cells and precious reagents. This offers users unparalleled flexibility with its independent, hands-free dispensing capabilities, enhancing the performance of multifaceted tasks on the one instrument.

The small footprint allows the 406FX to be easily integrated into existing laminar flow hoods, expanding its adaptability across diverse laboratory environments. The onboard touch screen on the 406 FX makes protocol creation simple and intuitive.

Maintaining the Agilent BioTek 406FX Washer Dispenser is easy due to the Ultrasonic Advantage feature which effectively cleans manifold tube build-up to prevent operational malfunctions.

The 406FX’s versatility and capabilities cater to a wide range of applications, from cell-based assays to ELISA, ELISpot, and Meso Scale Discovery (MSD) workflows.

InvivoGen introduces a new and improved Tet-on system, specifically designed for the controlled expression of cytotoxic proteins.

Conditional expression of proteins in mammalian cells is favoured over stable expression in a variety of basic and applied research areas, including functional genomics, gene therapy, biopharmaceutical protein production, and drug screening. Depending on experimental requirements, researchers can efficiently activate or suppress a gene of interest (GOI) at will, using different systems based on the E. coli Tn10-specified tetracycline-resistance operon.

InvivoGen offers such a system, named TiGer (Tetracycline-induced Gene expression or repression) system, which comprises two RepTor™ cell lines and two series of pTiGer plasmids (cloning and control vectors):

• RepTor™ cell lines: HEK293 and A549 cells constitutively expressing the tetracycline repressor protein (TetR)
• pTiGer-mcs plasmids:  Cloning of a gene of interest (GOI) downstream of a Tet-inducible promoter
• pTiGer-reporter plasmids: Tet-inducible expression of a reporter gene (controls)

The TiGer system has been specifically designed to allow the controlled expression of cytotoxic genes.

• Minimal GOI background expression without tetracycline
• Strong GOI expression with tetracycline

InvivoGen’s TiGer Tet-on system relies on the combined use of two plasmids; the first expressing an optimized TetR construct, and the second, pTiGer, a GOI under the control of an engineered tetracycline operator (tetO)-containing promoter. It features the strong composite CMV-EF1 promoter modified to integrate a tetracycline response element (TRE).

The TetR plasmid was stably transfected into the RepTor™ cell lines, to save you time and effort. Upon transfection of the RepTor™ cells with a pTiGer-GOI plasmid, TetR binds to TRE and blocks the transcription of the GOI [2]. Addition of doxycycline, a synthetic analogue of tetracycline, leads to the release of TetR from the tetO sequences, resulting in the derepression of the CMV-EF1-TRE promoter and transcription of the GOI.

The TiGer Tet-on system has been engineered to guarantee maximal repression of the GOI, ensuring minimal leakage of GOI expression in the absence of tetracycline/doxycycline, and strong GOI expression in the presence of the antibiotic. This key feature confers the TiGer Tet-on system with the advantage of working with cytotoxic genes (e.g. proteases, pore-forming proteins, gain-of-function variants).

InvivoGen’s TiGer Tet-on system is useful for, but not restricted to the following applications:

• Controlled expression of cytotoxic genes
• Fine-tuned expression of a GOI
• Screening of toxic protein inhibitors