HEK-Blue™-2 cells, engineered to become extremely sensitive, recognize even small amounts of mycoplasma through the Toll-like receptor (TLR) 2. This leads to the activation of NF-κB and the subsequent release of SEAP, a secreted embryonic alkaline phosphatase reporter protein, in the supernatant. These cells are cultured in HEK-Blue™ Detection medium. In the presence of SEAP, it changes its color to purple/blue, making it ideal for routine tests. The simple addition of test samples to these cells provides colorimetric results with sensitivity similar to luminescence-based biochemical assays. SEAP activity can also be measured using a spectrophotometer for a more precise and semi-quantitative result. Product Datasheet
