Bioconjugation

Bioconjugation is the technique of covalently binding molecules, at least one of which is a biomolecule such as a protein. These linked biomolecules serve functions ranging from drug delivery to imaging cellular functions. Bioconjugate synthesis involves optimisation of procedural design and material selection. The stability of the resulting molecule will need to be sufficient to maintain its chemical integrity throughout all further experimental processing, including permeabilisation and fixation if necessary. To retain empirical relevance, the conjugation procedure also must not compromise the essential functional properties of the unlabelled molecules.

Cell Structure and Organelle Dyes

AAT Bioquest’s extensive collection of organelle-selective dyes has been optimized for live and fixed-cell imaging applications. Available in a broad spectrum of colours, our organelle-selective dyes are compatible with a majority of laser and filter settings commonly equipped on most fluorescent microscopes and flow cytometers.