Agilent

Agilent xCELLigence Real-Time Cell Analysis (RTCA)

Label-free real-time cell analysis
Discover what you’ve been missing between endpoints. The Agilent xCELLigence real-time cell analyser (RTCA) harnesses impedance-based biosensor technology to continuously monitor cell health, behaviour, and function with high accuracy, sensitivity, and reproducibility. All without the use of labels and in real time.

The xCELLigence portfolio offers 9 different configurations with a range of throughputs (16-, 48-, 96- or 384-well formats) and functionalities. Perform quantitative monitoring of cardiomyocyte beating (milliseconds), receptor signalling (minutes), cell migration and invasion (hours), and cell growth and killing kinetics (hours/days) in real time without the use of labels. Experience robust assays, from discovery and process development to manufacturing quality control.

Agilent xCELLigence RTCA eSight

The xCELLigence RTCA eSight provides a simple, automated workflow that generates crucial data around the clock—all within your incubator. With real-time live cell analysis, and the combination of impedance (label-free) and imaging (brightfield, red, green, and blue fluorescent channels), you can easily monitor and quantify kinetic cellular data over the course of seconds, hours, or days.
Five cradles, supporting up to 96-well capability, ensure that the experience is multi-user friendly while securing a high-throughput, information-rich, and efficient workflow.

Multiplex impedance-based data with live cell imaging for increased confidence.
xCELLigence RTCA eSight adds live cell imaging for increased confidence in your cell analysis and conclusions. Go a step further in your research and easily monitor and quantify assays spanning immune cell activation, proliferation, clustering, potency, target cell killing, as well as viral CPE and drug discovery. Uncover unique drug mechanisms of action and cellular phenomena by multiplexing with both impedance and imaging—all in the same well. Capture precious cellular and biological events as they happen, and never miss a crucial time point again.

Agilent BioTek cell imagers and microscopes

Capture spectacular images, z-stacks, montages, and time-lapse sequences using different vessels, including Agilent cell culture and imaging microplates. These instruments support a wide range of microscopy workflows, including live cell kinetics.

• Capture with confidence
  Execute a wide range of widefield and confocal imaging applications including live- and fixed-cell biologies.
• Process and optimise images
  Image-processing tools provided by Gen5 software improve your final results, delivering publication-quality images.
• Analyse with certainty
  Use Gen5 to analyse and obtain a wealth of data on a population, single cell, or subcellular level.
• Publish without external software
  Automatically create scatterplots, histograms, and IC50/EC50 curves from generated data.

Agilent BioTek liquid handling and automation

Why use multiple instruments for washing and reagent dispensing?

With their small footprint, Agilent BioTek washers, dispensers, and combination washer dispensers are the ultimate in modularity. These affordable, compact instruments are designed to offer the best performance, low maintenance, ease of use, and powerful functionality.

Featured technologies

The Agilent BioTek Ultrasonic Advantage feature of the 406 FX washer dispenser automatically maintains clogfree dispense and aspirate tubes.

The Agilent BioTek MultiFlo FX offers automated media exchange (AMX) technology to provide gentle media exchange for cells and spheroids.

Modularity enables application versatility
Agilent BioTek liquid handling and automation instruments are designed for ultimate versatility, with modules that enable expanded applications as your research needs change. The wide range of applications includes:

• Immunocytochemistry
• Magnetic bead assays
• Automated media exchange for 3D cell structures
• High-content screening
• Cell seeding
• ELISA

Agilent BioTek microplate readers

Configurable Agilent BioTek microplate readers offer a range of modules, options, and accessories to address low, medium, and ultrahigh throughput applications. Absorbance readers deliver excellent performance and functionality from basic ELISA to advanced detection chemistries. Multimode readers support monochromator-based, filter-based, and combination methods-enabling detection of UV-Vis absorbance, luminescence, fluorescence, fluorescence polarisation, time-resolved fluorescence (TRF), FRET, and Alphascreen assays.

Applications in 6- to 1536-well microplates are accommodated, depending on the selected microplate reader.

Agilent BioTek Gen6 data analysis software – with many preprogrammed assays – makes plate setup, reading, and analysis seamless and efficient, so you can move on with your research.

Agilent NovoCyte flow cytometers

Built on a proven platform, NovoCyte Penteon, NovoCyte Quanteon, and NovoCyte Advanteon flow cytometers deliver expanded capabilities that accommodate today’s sophisticated, multicolour flow cytometry assays. Put more flow into your flow cytometry with features such as:

• High-sensitivity and resolution
• Smart-design functionalities and walkaway convenience
• Automation-ready capabilities for high-throughput needs
• Wide, seven-log dynamic range that eliminates the need for routine detector adjustments
• Side-scatter resolution of 100nm, which allows for small particle detection
• Exceptional fluidic stability and precision volumetric measurement for absolute counts in every sample