About Norgen Biotek
Norgen Biotek is committed to providing their customers with first class sample preparation kits – including isolation and purification kits, sample collection and preservation. They have products for Liquid biopsy, Stool microbiomes, Saliva, Urine and Exosome workflows.
Liquid Biopsy Plasma/Serum Workflow
Preservation
cf-DNA/cf-RNA Preservative Tube
Norgen’s cf-DNA/cf-RNA Preservative Tubes provide an easy method of cf-DNA, cf-RNA and CTC preservation from whole blood samples, while also minimising hemolysis during shipping and storage. The tubes provide cf-DNA and cf-RNA preservation for 30 days at room temperature, and CTCs preservation for 14 days at room temperature, with no cold-chain transportation or storage required. Purified cf-DNA and cf-RNA are compatible with any downstream application, including PCR, qPCR, rt-qPCR, gene expression analysis, microarrays and Next-Generation Sequencing.
Isolation
- Plasma/Serum cfc-DNA Purification Kits – Isolate all types of circulating DNA, including ct-DNA and featal DNA
- Plasma/Serum cfc-DNA/cfc-RNA Advanced Fractionation Kit – Isolate both cf-DNA and cf-RNA into two separate elutions
- Plasma/Serum RNA Purification Kits – Isolate total cell-free RNA from the sample, including circulating and exosomal
- Plasma/Serum Exosome and Free-Circulating RNA Isolation Kits – Isolate circulating RNA and exosomal RNA into two separate elutions
Downstream applications
Next Generation Sequencing
Featured Publication
Stool Workflow Solutions
Preservation
Stool Nucleic Acid Collection and Preservation Tube
Norgen’s Stool Nucleic Acid Collection and Preservation tubes provide an easy and rapid method of collecting and preserving DNA and RNA from stool samples, while also maintaining the microbiome profile in the stool samples as close to fresh as possible. The tubes preserve DNA for 2 years and RNA for 7 days in stool samples without any cold-chain transportation or storage required. Purified high-quality DNA and RNA are compatible with any downstream applications, including PCR/qPCR/rt-qPCR, metagenomics, metatranscriptomics, gene expression analyses, and NGS.
Isolation
- Stool DNA Isolation Kits – Isolate both host and microbial DNA from stool
- Stool Nucleic Acid Isolation Kit – Isolate both DNA and RNA from stool
- Stool RNA Purification Kit – Purify total RNA from stool
Downstream applications
Metagenomics Sequencing
Featured Publication
Human Stool Preservation Impacts Taxonomic Profiles in 16S Metagenomics Studies.
Urine Workflow Solutions
Preservation
Urine Collection and Preservation Devices
Norgen’s Urine Collection and Preservation Devices provide a rapid and simple method for the collection, preservation and shipping of urine samples. The Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. Nucleic acids and proteins are preserved for more than 2 years at room temperature. Compatible with most DNA/RNA isolation methods.
Isolation
From Whole Urine:
- Total RNA – Purify high quality total RNA of all sizes from urine
- Total DNA – Isolate high quality total DNA of all sizes from urine
- Cell Pellet – Isolate high quality RNA or DNA from exfoliated cells or bacteria from urine
From Cell-free Urine:
- cf-RNA – Purify high quality cell-free circulating and exosomal RNA from urine
- cf-DNA – Isolate high quality cell-free circulating DNA from urine
- cfc-Nucleic Acids – Purify high quality cell-free circulating DNA and cell-free circulating RNA from urine
- Exosome – Purify high quality intact exosomes from urine
Downstream applications
Next Generation Sequencing
Featured Publication
Extracellular circular RNA profiles in plasma and urine of healthy, male college athletes
Saliva Workflow Solutions
Preservation
Saliva RNA Collection and Preservation Devices
Norgen’s Saliva RNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of RNA in saliva samples at ambient temperature. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for RNA isolation and analysis. The Saliva RNA in preserved samples is stable for up to 2 months at room temperature. This kit is ideal for collecting and preserving RNA samples for epidemiological and population studies.
Saliva DNA Collection and Preservation Devices
Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat# RU45400). Each of Norgen’s Collection Tubes is labelled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for epidemiological and population studies.
Isolation
- Saliva DNA Isolation Kits – Rapidly purify high-quality DNA from preserved and fresh saliva samples.
- Saliva RNA Isolation Kits – Rapidly purify total RNA from preserved and non-preserved saliva and nasal throat/swabs
Downstream applications
Next Generation Sequencing