Agilent BioTek

BioTek is now Agilent. The Agilent BioTek family of microplate instrumentation and software is the worldwide leader in these solutions. The family includes cell imaging and analysis systems, microplate readers, washers, dispenser and automated incubators. Agilent's BioTek products are used in life science research, drug discovery, clinical and industrial applications. The products have won numerous awards and are backed by over 50 years of experience in the technologies.

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Flow Cytometer Systems

NovoCyte Advanteon Flow Cytometer Systems 1-3 Lasers

The NovoCyte Advanteon flow cytometer builds upon the highly successful NovoCyte, and NovoCyte Quanteon. The system provides an advanced set of capabilities for the most demanding scientists, yet is remarkably simple to operate. The NovoCyte Advanteon can accommodate high-end and increasingly sophisticated multicolor flow cytometry assays. The system offers flexibility with 1, 2, or 3 laser options, up to 21 fluorescence channels, and 23 independent detectors.

The NovoCyte Advanteon is customizable to meet your specific needs but is easily upgradeable to meet your future demands. When throughput is essential, the NovoSampler Q can be integrated into different laboratory automation platforms. The sampler can efficiently process both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. For the NovoCyte Advanteon, the intuitive and industry honored NovoExpress software is now even more advanced, providing an exceptional user experience in data acquisition, analysis, and reporting.

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NovoCyte Flow Cytometer Systems 1-3 Lasers

NovoCyte is a high-performance benchtop flow cytometer designed for all levels of users and all types of laboratories. The budget-friendly instrument can detect up to 17 parameters with enhanced sensitivity and resolution. The customizable laser and optical configurations of the NovoCyte offer a high degree of flexibility while providing complex cell analysis capabilities.

The NovoExpress software facilitates easy and intuitive sample acquisition and analysis. Automation of multiple fluidic functions eliminates cumbersome and time consuming procedures. Manual sample handling is minimized by the flexible NovoSampler Pro. The system can automatically analyze samples in single tubes, multi-tube racks, or 24-, 48-, or 96-well plates

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NovoCyte Penteon Flow Cytometer Systems 5 Lasers

The NovoCyte Penteon flow cytometer builds on its successful predecessor, the NovoCyte, and provides an expanded set of capabilities that accommodate today's high-end and increasingly sophisticated multi-color flow cytometry assays. You now have the flexibility to choose from up to 30 fluorescence channels utilizing up to 5 lasers with up to 30 independent detectors. The NovoSampler Q can be integrated into different laboratory automation platforms and efficiently processes both FACS tubes (using a 40-tube rack) and 24-, 48-, 96-, and 384-well plates. The intuitive and industry leading NovoExpress software has been further advanced, providing an exceptional user experience in data acquisition, analysis and reporting.

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NovoCyte Quanteon Flow Cytometer Systems 4 Lasers

The NovoCyte Quanteon is a new generation in the successful line of NovoCyte flow cytometers. The Quanteon is a 4 laser flow cytometer that can be configured with up to 25 independent photomultipliers for meeting the most demanding sample panels. The proprietary photomultiplier technology employed in the Quanteon provides excellent sensitivity, stability, and a 7.2 log dynamic range. Very dim signals and very bright signals can all be captured and gated in the same view, therefore eliminating the need for laborious trial and error PMT tuning procedures.

When developing large color panels, the Quanteon saves hours of setup and analysis time. Excellent fluidics provide steady and consistent sample delivery, with high reproducibility and exceptionally low CVs. Absolute counts, without the use of expensive beads, will save you time and money.

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Flow Cytometry Automation

NovoSampler Pro

The NovoSampler Pro is compatible with 24-, 48-, and 96-well plate configurations. The sampler can accommodate 24 tube racks as well. Load your plate and let the system record the data for you.

Walk away convenience delivers on time savings and cost savings in the laboratory. Flexible sampling of microplates allows for small projects or high-throughput, 24/7 workflows. Low carryover, and orbital shaking allow you to maximize your efficiency and make the instrument work for you.

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NovoSampler Q

The NovoSampler Q is compatible with 24-, 48-, 96-, 384- well plate configurations. It can accommodate 24 and 40 tube racks as well. Automation tools for sample handling and integrated software deliver on a workflow with minimal manual intervention.

Walk away convenience delivers on time savings and cost savings in the laboratory. Flexible sampling of microplates allows for small projects or high-throughput, 24/7 workflows. Low carryover, orbital shaking, and embedded barcode reader ensure that your samples are processed quickly and accurately with results you can trust.

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Robotic Automation

The robotic arm compatible with flow cytometry systems features a precise grip and handle with high quality grippers and fingers for consistent and reliable sample handling. Automation offers simplicity and walk-away operation. Time consuming sample handling and error prone practices are eliminated with robotic automation.

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NEW - Agilent BioTek Cytation C10 Confocal Imaging Reader

Cytation C10 Applications

Lisa's List - 5 tips to get the most out of your plate washer

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Gen5 Software Features for Imaging & Microscopy

  • Agilent BioTek Gen5 Software for imaging and microscopy offers automated image capture, processing and analysis for a broad range of samples, from whole organism imaging to high magnification subcellular imaging.
  • Raw data and images are transformed into meaningful results with powerful built-in capture, process and analysis tools.
  • The automated process, known as Augmented Microscopy , enables simple and straightforward imaging and analysis workflows.

Gen5 Software Features for Detection

  • Gen5 software for Agilent BioTek multimode and single mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning and well area scanning.
  • It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications.
  • Gen5 microplate reader software is also used to integrate Agilent BioTek plate readers to Agilent BioTek BioStack and other automated systems.

Liquid Handling Control Software

  • Liquid Handling Control (LHC) software is a powerful yet flexible interface for use with washers, dispensers, and combination liquid handling systems.
  • LHC software makes it easy to program even the most complex protocols with a choice of deployment.
  • These protocols can be run directly from the computer or downloaded to the instrument for convenient onboard recall and operation.

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Agilent BioTek Instruments, Accessories & Peripherals

Confocal Imaging Reader


  • Compact, affordable confocal imager for every laboratory
  • Confocal: Improved image quality and analysis
  • High quality optical components
  • Confocal imaging and multi-mode plate reader in one
  • Confocal plus widefield = stunning images and analysis
  • Environmental controls for live cell imaging
  • Hit-picking: Imaging + multi-mode detection saves time and data storage
  • Variable bandwidth for sensitivity and specificity
  • Cytation C10 is Ready for Any Assay

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Cell Imaging Multi-Mode Readers

Agilent BioTek Cytation 7 Cell Imaging Multi-Mode Reader

Agilent BioTek Cytation 7 cell imaging multimode reader combines automated digital upright and inverted widefield microscopy with conventional multimode microplate reading. Cytation 7 includes continually variable bandpass monochromators for versatility and performance for general multimode plate reader applications. The inverted microscopy module provides sample visualization and the upright microscopy module with reflected light imaging enables even more applications, including ELISpot and fast slide scanning. Cytation 7 is controlled by Gen5 software, which combines ease-of-use with powerful processing and analysis capabilities.

Agilent BioTek Cytation 5 Cell Imaging Multi-Mode Reader

Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. The multimode detection modules include filter- and monochromator-based fluorescence detection, luminescence, and UV-Vis absorbance detection. Gen5  software provides complete control over all imaging and data capture, plus powerful image and data analysis.

Agilent BioTek Cytation 1 Cell Imaging Multi-Mode Reader

Agilent BioTek Cytation 1 cell imaging multimode reader combines fluorescence and high contrast brightfield imaging with conventional multimode detection. This affordable imaging reader delivers performance and economy compared to other digital microscopy systems. The Cytation 1 design provides both quantitative phenotypic cellular data with well-based quantitative data. The multimode detection module has high sensitivity filter-based fluorescence and a monochromator for UV-Vis absorbance. Temperature control and shaking are standard. CO2 /O control and reagent injectors are available. Gen5 software simplifies image capture and plate reading.

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Automated Cell Imagers

Agilent BioTek Lionheart FX Automated Microscope

Lionheart FX automated microscope is a compact system for a broad range of imaging workflows. It offers up to 60x air; 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40 °C and effective containment for CO2 /O2  control. A humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays.

Agilent BioTek Lionheart LX Automated Microscope

The Lionheart LX automated microscope is designed for affordability, offering an alternative to expensive, custom-built automated microscopes that are complex to learn and use. The compact, ocular free hardware design is more comfortable to use and reduces ergonomic distress in operators. Along with Gen5  software, Lionheart LX enables Augmented Microscopy , which fully automates image capture, processing, and analysis. Operation is easy, load samples, start a run and gather publication-ready images and quantitative data. Lionheart LX can increase your research output and reduce time and costs.

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Multi-Plate Live Cell Imaging System

Agilent BioTek BioSpa Live Cell Analysis System

The BioSpa Live Cell Analysis System offers unique benefits for a wide variety of live cell imaging applications:

  • Brightfield, high contrast brightfield, color brightfield, phase contrast and fluorescence imaging offers amazing imaging application versatility on a single platform. Confocal imaging available when integrated with Cytation C10
  • 1.25x to 60x magnification captures the level of detail you need, from whole well montage to intracellular detail
  • Integrated temperature and CO2/O2 control, plus humidity monitoring support live cell assays
  • 6 objectives on an automated turret provides effortless image capture at various magnifications
  • Z-stacking and z-projection, plus z-stacked montage are powerful capture and processing tools to get vital information from 3D biology
  • Patented laser autofocus provides increased reproducibility, while decreasing phototoxic and photobleaching effects
  • Advanced cell-level analysis like nuclear and cytoplasmic masking define critical, information-rich regions of interest
  • Automated washing, reagent dispensing and media replacement are easy add-ons, bringing efficiency to critical cell sample prep and maintenance throughout the entire workflow
  • The BioSpa Live Cell Analysis System easily fits inside commercially available containment enclosures and biosafety cabinets

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Imaging & Microscopy Peripherals

Agilent BioTek's peripheral products help to expand your instrument applications and improve workflow efficiency. From automated incubators to micro-volume plates, we have the peripherals to help advance your research.

AutoScratch Wound Making Tool

For use with:Lionheart FXCytation 7Cytation 5Cytation 1Scratch Assay App

Agilent BioTek AutoScratch wound making tool automatically creates reproducible scratch wounds in cell monolayers grown in 24- or 96-well microplates for cell migration and invasion studies. AutoScratch automates the sample prep for imaging using BioTek Cytation  cell imaging multimode readers and Lionheart  automated microscopes. The Scratch Assay App automates image collection and analysis of wound width, wound confluence and maximum wound healing rate.

Cell Count & Viability Starter Kit

For use with: Cell Count & Viability AppLionheart FXCytation 5

Agilent BioTek's Cell Count & Viability Starter Kit includes everything a researcher needs to count cells and measure viability in a mammalian cell suspension using a phase contrast-equipped BioTek  Cytation Cell Imaging Multi-Mode Reader or a Lionheart Automated Imager. The Kit saves time and increases data quality by automating the tedious and error-prone process of mammalian cell counting.

Other Peripheral products:

Multi-Mode Microplate Readers

Agilent BioTek Synergy Neo2 Hybrid Multi-Mode Reader

The Agilent BioTek Synergy Neo2 multimode microplate reader features patented Hybrid Technology, with independent optical paths that ensure excellent performance in all detection modes. Variable bandwidth quad monochromators, sensitive high transmission filter-based optics, laser TRF, and up to 4 PMTs provide ultrafast measurements. Advanced environmental controls, including CO2 /O2  control, incubation to 70 °C, and variable shaking support live cell assays. BioStack Neo delivers walk-away automation, high throughput, barcode-labeled filter cubes to streamline workflows and limit errors. Powerful Gen5 software provides complete reader control, powerful data analysis, and LIMS integration.

Agilent BioTek Cytation Hybrid Multi-Mode Reader

Cytation Cell Imaging Multi-Mode Readers are uniquely designed to support applications across automated digital microscopy and conventional multi-mode detection. Here we highlight a few key application areas.

  • Cell based assays
  • Biomarker assays
  • Imaging applications
  • Protein quantification
  • Kinetic assays
  • Micro-volume nucleic acid and protein quantification with the Take3 Micro-Volume Plate

Agilent BioTek Synergy H1 Hybrid Multi-Mode Reader

Agilent BioTek Synergy H1 is a modular multimode microplate reader, with monochromator-based optics and filter-based optics. The patented Hybrid Technology  offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change. Synergy H1 offers continuously variable bandwidth monochromators for fluorescence excitation and emission wavelength selection. Fluorescence bandwidth can be set between 9 and 50 nm, in 1 nm increments, allowing users to fully optimize reader settings to drive the best assay performance compared to fixed bandwidth systems.

Agilent BioTek Synergy HTX Multi-Mode Reader

The Agilent BioTek Synergy HTX is a compact, economical multimode reader for 6- to 384-well microplates and Take3 microvolume plates. The unique dual optics design provides superior performance for UV-Vis absorbance, fluorescence, luminescence, and AlphaScreen/AlphaLISA workflows. With incubation and shaking, plus a dual reagent injector module, the system will meet all your assay requirements now and in the future. Synergy HTX is controlled by the easy-to-use, yet powerful, Gen5 software for data collection, analysis, exporting, and reporting.

Agilent BioTek Synergy LX Multi-Mode Reader

The Agilent BioTek Synergy LX multimode reader automates many common microplate assays. The high-quality optic design ensures excellent data in absorbance, fluorescence, and luminescence detection modes. This affordable system is ideal for many common assays including nucleic acid and protein quantification, ELISA, BCA, Bradford, and cell viability. The data display, output to a USB flash drive, printer, or Gen5 software make Synergy LX a versatile assay workstation. The large touch screen user interface and onboard software simplify programming and save time in operation of microplate and microvolume assays.

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Multi-Plate Absorbance Reader

Agilent BioTek LogPhase 600 Microbiology Reader

Only 4-plate microplate reader on the market. Keep your cells in suspension for optimal growth. Condensation and evaporation control. Consistent growth conditions = consistent data. Easy to use and powerful targeted app

Absorbance Microplate Reader

Agilent BioTek Epoch 2 Microplate Spectrophotometer

The Agilent BioTek Epoch 2 microplate spectrophotometer delivers excellent performance for UV-Vis absorbance measurements. Measurements can be performed in 6- to 384-well microplates, cuvettes, and in microvolume samples with the available Take3 microvolume plate. The broad wavelength range enables applications from nucleic acid and protein quantification in the low UV to microbial growth assays higher wavelengths. Epoch 2 is easy to use and the optional touch screen interface makes it simple to choose predefined protocols or to define custom programs. Endpoint, kinetic, spectral scanning and well area scanning modes, plus incubation and shaking enable wide-ranging applications.

Agilent BioTek Epoch Microplate Spectrophotometer

Epoch microplate spectrophotometer has monochromator-based optics, for a filter-free, wide wavelength range for UV-Vis absorbance measurements. These measurements can be done in many microplate formats and in 2 µL samples, when the available Take3 microvolume plate is used. Epoch is controlled with the Gen5 software interface, with simple programming and powerful data analysis. This robust, low maintenance microplate absorbance reader is a cost-effective system. Reliability and ease of use ensure time savings in laboratory workflows.

Agilent BioTek 800 TS Absorbance Reader

The Agilent BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. The color touch screen provides a visual user interface, making programming fast and intuitive. Expanded functionality and data analysis is available with Gen5  software. The 800 TS partners with the 50 TS  washer, making a compact, versatile, and affordable system to automate many application workflows.

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Multi-Mode Washer Dispensers

Agilent BioTek EL406 Washer Dispenser

The Agilent BioTek EL406 washer dispenser is a compact instrument offering fast, full plate washing along with three reagent dispensers. Combines fast microplate washing with 3 reagent dispensers in 1 compact instrument. 96-, 384- and 1536-well plate compatible. Gentle processing for cell-based assays. Bead washing: magnetic and polystyrene. Easy maintenance with Ultrasonic Advantage

Agilent BioTek MultiFlo FX Multi-Mode Dispenser

MultiFlo FX is an automated multimode reagent dispenser for 6- to 1536-well microplates. The system incorporates several unique technologies in its modular design, such as Parallel Dispense, random access dispense (RAD) and patented Automated Media Exchange (AMX) modules. The technologies in MultiFlo FX microplate dispenser enable many liquid handling applications from 2D and 3D cell culture to concentration normalization assays, ELISA, bead-based assays and more. A fully configured MultiFlo FX replaces up to five liquid handlers, saving space, time, and instrumentation budgets. MultiFlo FX delivers complete workflow automation in cell imaging and biochemical applications.

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Microplate Washers

Agilent BioTek 405 TS Washer

The Agilent BioTek 405 TS washer has an excellent reputation in life science research for outstanding results in many applications. The 405 TS has a unique set of features designed to efficiently wash cell-based assays, microsphere-based assays, and ELISA applications. Programming and operation via the touchscreen interface is intuitive and simple. The patented Verify technology automatically checks for manifold tube blockages before a wash cycle, and Ultrasonic Advantage automatically cleans any blocked manifold tubes. These functions save time and eliminate frustration while ensuring successful results.

Agilent BioTek 405 LS Washer

The Agilent BioTek 405 LS washer has a well earned reputation as an economical plate washer that offers excellent performance and reliability. Washing protocols range from gentle cell washing to vigorous ELISA workflows. The 405 LS has a simple user interface for creation and execution of wash, dispense, aspirate, and maintenance protocols within the extensive onboard software. For use under computer control, Agilent BioTek LHC  software used with the 405 LS is available in standard and 21 CFR Part 11 compliant versions. The optional Agilent BioTek BioStack  is available for walk-away automation of up to 50 microplates.

Agilent BioTek ELx405 Select Deep Well Washer

The Agilent BioTek ELx405 Select is the first deep well plate washer that aspirates and dispenses into 96- and 384-well deep well and standard height microplates. The ELx405 Select is available in several configurations to address a wide variety of plate washing applications, including ELISA and cell-based assays. Maintenance is easy with the available patented Ultrasonic Advantage  that automates and cleans manifold tubes to remove built-up salt and protein. Ideal for cell culture, plasmid DNA purification and much more.

Agilent BioTek 50 TS Washer

The Agilent BioTek 50 TS washer offers excellent microplate washing performance. Ideal for use as an ELISA washer, the system is also well suited for cell-based assay washing. The 50 TS can process biomagnetic or polystyrene bead and vacuum filtration protocols as well. The color touch screen provides a visual interface that makes creating protocols fast and intuitive. When used with the 800 TS  absorbance reader or other detection system, the 50 TS delivers convenience and high-quality results to your laboratory plate washing workflows.

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Microplate Dispensers

Agilent BioTek MultiFlo FX Multi-Mode Dispenser

MultiFlo FX is an automated multimode reagent dispenser for 6- to 1536-well microplates. The system incorporates several unique technologies in its modular design, such as Parallel Dispenserandom access dispense (RAD)  and patented Automated Media Exchange (AMX)  modules. The technologies in MultiFlo FX microplate dispenser enable many liquid handling applications from 2D and 3D cell culture to concentration normalization assays, ELISA, bead-based assays and more. A fully configured MultiFlo FX replaces up to five liquid handlers, saving space, time, and instrumentation budgets. MultiFlo FX delivers complete workflow automation in cell imaging and biochemical applications.

Agilent BioTekMicroFill Dispenser

MicroFill is an economical, compact, and reliable dispenser for 24-, 96-, and 384-well microplates. An autoclavable fluid path is ideal for applications that require sterility. The dispense flow rates are easily selected to accommodate sensitive cell-based assays or vigorous dispensing for ELISA.;Accurate and precise dispensing are guaranteed, without the time-consuming recalibration, cassette replacement, and maintenance commonly associated with other dispensers. Intuitive, on-board software is easy to use and saves time in instrument setup.

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Agilent BioTek BioSpa 8 Automated Incubator

  • Complete workflow automation, sample prep to detection
  • Temperature, CO2/O2 control; humidity monitoring
  • Records environment conditions; automated alerts
  • OnDemand mode: Remove/replace labware, independent scheduling and multi-user profiles
  • Compact for biosafety cabinet use
  • Uncomplicated software and simple integration

Agilent BioTek BioStack Microplate Stacker

  • De-lidding and re-lidding microplates with fast plate exchange times
  • Ultra-fast plate transfers with BioStack Neo
  • Convenient re-stacking for complete automation of endpoint and kinetic processes
  • 10, 30 and 50 plate removable, exchangeable stacks
  • Super compact footprint saves bench space - and can fit in biosafety enclosure

Agilent BioTek BenchCel Microplate Handler

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