The revolutionary ImageStream®X Mk II Imaging Flow Cytometer combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy. This unique combination enables a broad range of applications that would be impossible using either technique alone.
The ImageStreamX Mk II System is a benchtop, multispectral, imaging flow cytometer designed for the acquisition of up to 12 channels of cellular imagery. By collecting large numbers of digital images per sample and providing a numerical representation of image-based features, the ImageStreamX Mk II combines the per cell information content provided by standard microscopy with the statistical significance afforded by large sample sizes common to standard flow cytometry. With the ImageStreamX Mk II System, fluorescence intensity measurements are acquired as with a conventional flow cytometer; however, the best applications for the ImageStreamX Mk II take advantage of the system's imaging abilities to locate and quantitate the distribution of signals on in or between cells.
Features:
Fast: Can analyse up to 5,000 cells per second with real-time intensity compensation. Ideal for rare cell analysis.
Easy-to-use: The intuitive user interface provides real-time plotting and graphical gating, as well as images of every cell. The easy to use compensation wizard quickly guides you through set up of multi-colour compensation matrices.
Flexible: Accepts up to 7 lasers and works with sample volumes of 20-200 uL for added experimental flexibility. Perfect for multi-user laboratories.
Efficient: Fluidics that provide up to 95% sample utilisation when every cell is required. Recover unused sample for further analysis when orthogonal methods are required.
Adaptable: The modular design allows the system to grow with your research needs and budget through a broad range of field-installable options.
Research Areas:
Immunology
Oncology
Biochemistry
Drug Discovery
Stem Cell Biology
Hematology
Microbiology
Virology
Nanotechnology
Toxicology
Oceanography
Parasitology
Options
Laser Options
An Expandable Fluorescent Palette
The standard 488nm laser of the ImageStreamx MkII Flow Cytometer may be augmented with up to six additional lasers, including 375, 405, 561, 592, and 642. A high-power 488nm laser upgrade is also available for enhanced detection of fluorescent dyes excited at that wavelength.
Features:
Increase experimental flexibility by utilising a broader palette of fluorescent markers
Improve data quality by employing more spectrally distinct dyes, thereby reducing crosstalk
Improve throughput via multiplexing of experiments in a single tube or well
Additional laser options can be installed in the field by certified technicians
AutoSampler
AutoSampler for Multiwell Plates
The AutoSampler option for the ImageStreamx MkII Flow Cytometer enhances productivity with unattended sample loading from multiwell plates. The fully integrated AutoSampler option allows you to easily perform dose response and time course studies even in primary samples, due to the system's capacity for imaging large numbers of cells from each sample.
Features:
Ideal for high throughput analysis of suspended cells
Easy to use acquisition templates allow for multiple experiments per plate
Unattended operation and in-line bubble detection enables robust operation
Automatic process logging and error notification
Automatic sipper rinse between samples and <0.5% carryover
Automatic sample resuspension via aspiration
Advanced template function allows groups of wells to be defined for flexible plate configuration
Automated data analysis
Field installable by certified technicians
12 Image Channels
Up to 12 Images of Every Cell
The 12 Image Channel option significantly expands the experimental flexibility and analytical potential of the ImageStreamx MkII Flow Cytometer by doubling the number of images per cell from six to twelve. This option is comprised of a second image detection system, including a filter wheel, inline filters, spectral decomposition assembly and a proprietary charge-coupled device (CCD) camera.
Features:
Increase experimental capacity by utilising a broader palette of fluorescent markers
Improve data quality by reducing or eliminating spectral crosstalk between detection channels
Perform functional assays of immunophenotypically-defined subpopulations
Improve throughput via multiplexing of experiments in a single tube or sample well
Field installable by certified technicians
MultiMag
60X/40X/20X Magnification
The MultiMag option provides 20X and 60X objective lenses, in addition to the standard 40X objective, for greater experimental flexibility and improved resolution. The 60X objective lens increases magnification for small objects, such as yeast and bacteria, and offers greater detail with mammalian and plant cells. The 20X objective lens provides and increased field of view for the imaging of large cells, such as epithelial cells.
Features:
Superior quality, high numerical aperture (NA) objectives yield crisp images
Convenient magnification changes via motorised objective mount
Crisp focus is ensured by advanced, patented auto focus technology
Extended Depth of Field
A unique optical system upgrade for the ImageStreamx MkII Flow Cytometer keeps the depth of cell in focus without loss of fluorescence sensitivity. No you can image FISH spots or other sub-cellular features, no matter where they lie int he cell.
Videos
FlowSight
Compact Flow Cytometer with Vision
The FlowSight Imaging Flow Cytometer provides exceptional sensitivity, as well as images of every cell in up to 10 fluorescent channels at up to 4,000 events per second. A proprietary CCD camera, coupled with patented detection technology, yields fluorescence sensitivity that is at least ten times that of PMT-based flow cytometers across all channels.
Features:
Capable: Sensitive and flexible for ever need
Intuitive: Easy to use, with imagery for every cell
Visual: Quantitative imaging
Affordable: Designed and priced for every lab
Options
Excitation Lasers
Adding excitation lasers increases experimental flexibility by permitting a broader palette of fluorescent markers. The standard 488nm blue laser of the Flow Sight System may be augmented with up to three additional lasers at 405nm (violet), 561nm (green), and 642nm (red) wavelengths. The intensity of any laser may be adjusted to adapt to protocol needs.
AutoSampler
The fully integrated autoSampler option for the FlowSight enhances productivity with unattended sample loading from 96-well plates, and significantly facilitates dose-response and time-course studies.
Videos
CellStream
Ultra-high Sensitivity and Flexibility
The Amnis® CellStream® benchtop flow cytometry system from Luminex is a highly-customisable and compact flow cytometer that is the first to use a camera for detection. Only recently has the importance of extracellular vesicles as key mediators of intercellular communication been appreciated.
Features:
Unparalleled Sensitivity: The CellStream Flow Cytometry System delivers high fluorescence sensitivity with MESF <10 for FITC and MESF <5 for PE
1 to 7 Laser Capacity: The CellStream System can be configured with 1 to 7 lasers. Laser upgrades are easily performed in the lab with no downtime.
Unique Event Gallery Software Feature: This unique camera-enabled feature of the CellStream Acquisition Software allows for population verification, aids in troubleshooting, and resolves doublets.
Standard 96-well Plate AutoSampler: All CellStream Systems are equipped for both single tubes and 96-well plates. On-board fluidics handle all mixing and rinsing steps to give you walkaway, high throughput acquisition
Patented Optics Technology: The CellStream Flow Cytometry System contains patented time delay integration (TDI) and camera technology to rapidly capture low-resolution cell images and transform these into high throughput intensity data.
Imaging Flow Cytometer
Compact Flow Cytometer with Vision
Ultra-high Sensitivity and Flexibility
