Kapa - Sample QC

Ensuring quality in next generation sequencing results

Roche Sequencing Solutions offers a qPCR-based quantification and quality control kit for use with human genomic DNA samples. It combines the high performance of the KAPA SYBR FAST qPCR Master Mix with high-quality standards and allows for reliable assessment of concentration and quality with a single assay. The information gathered from this assay may be used to establish quality control minimums and optimize library construction parameters.

KAPA hgDNA Quantification and QC Kit

pdp_ban_hgdnaquantandqc_right.pngThese kits contain all the consumables needed for the qPCR-based quantification and quality assessment of human genomic DNA samples prior to NGS library construction. Each kit contains KAPA SYBR FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR through our Directed Evolution Technology, a set of pre-diluted DNA standards and primer premixes targeting different portions of a highly conserved single-copy human locus. Absolute quantification is achieved with the primer pair defining the shortest fragment and the additional primers providing information about the amount of amplifiable template in the DNA sample. Quality scores (or Q-ratios) generated with the kit may be used to predict the outcome of library construction or tailor workflows for samples of variable quality, particularly FFPE DNA.

Benefits

  • Quantification and quality assessment with a single assay.
  • Ability to correlate quality scores to sequencing metrics.
  • A versatile and easy-to-automate workflow.
  • Reduce time and effort by eliminating library construction and sequencing of samples that do not pass QC.



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Enquiry Form

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